Basic cell culture instructions

Some volumes that
Do NOT need to be exact in cell culture

1. Rinsing volume of PBS (as long as it fits in the dish and is sufficient to rinse the serum).

2. Volume of trypsin EDTA as long a bottom of plate or flask can be covered.

3. Volume of media used to resuspend your cells. The same number of cells will be there despite the volume of media used.
• Too little resuspension media will result in very high cell count and would require more dilution (and higher dilution factor). The volume needed to seed your next plate would then be very small, maybe too small to work with.
• Too much media would result in low cell count/ml and you may need a large volume to add to your new plate.
  
  
4. Volume of cells removed for cell counting.
• You want enough to work with, but not take all of your cells from your plate. If you want a dilution factor of 2, just add an equal amount of trypan blue.
  
  
5. Exact # of cells to be plated

• If you want to plate 2 x 10 5 cells onto your plate, but you have 2.1 x 10 5 cells/ml, plating 1ml will be easier than plating .953 ml.